In silico and multi-dimensional functional interrogation of tissue Tregs reveals novel therapeutics for autoimmunity and inflammation

Abstract Many inflammatory and autoimmune diseases present with specific organ manifestations. Understanding the immune process at site of inflammation may offer important insights into novel therapeutic strategies. To this end, we established a biology platform that allows us to develop deep understanding human tissue Treg behavior enable next generation therapeutics aimed activating these regulatory circuits location where disease manifests. We apply single-cell RNA sequencing computational tools our proprietary collection healthy diseased tissues generate high-resolution map immune-regulatory pathways. Based on learnings, in vitro culturing conditions induce expression pathways primary Treg. Lastly, have developed suite relevant phenotypic assays for multi-dimensional functional interrogation mapped vitro-conditioned This combination complex silico translational identify nodes forms foundation growing pipeline new class Treg-focused immune-mediated diseases.